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Measurement of the activity of TrxR from Caco-2/TC7 lysates (A) and ROS generation (B) on undifferentiated Caco-2/TC7 cells upon incubation with [Au­(L1)­JP] ( 1 ) at its IC 50 value for 24 h. ** p < 0.01 vs negative control.

Journal: Inorganic Chemistry

Article Title: Dual-Action NSAID-Gold(I) Alkynyl Hybrids for Synergistic Anti-Inflammatory and Anticancer Therapy of Colorectal Cancer

doi: 10.1021/acs.inorgchem.5c05908

Figure Lengend Snippet: Measurement of the activity of TrxR from Caco-2/TC7 lysates (A) and ROS generation (B) on undifferentiated Caco-2/TC7 cells upon incubation with [Au­(L1)­JP] ( 1 ) at its IC 50 value for 24 h. ** p < 0.01 vs negative control.

Article Snippet: We evaluated the enzyme activity in protein extracts from Caco-2/TC7 cells treated with the complex for 24 h. The intracellular TrxR activity was determined using a thioredoxin reductase colorimetric assay kit, which detects the reduction of DTNB (5,5′-dithio-bis (2-dinitrobenzoic acid); Ellman’s reagent) by the enzyme and NADPH to TNB (5-thio-2-nitrobenzoic acid) at 405 nm.

Techniques: Activity Assay, Incubation, Negative Control